Micronutrients: Part 1

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In this series of contributor articles, Dr. NPK of Elite Garden Wholesale begins his explanation of micronutrients in hydroponic systems. The original article can be found here.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.


Nothing Micro About Their Impact!


Hi everybody! Welcome to this installment of “let’s talk about elements”! This post is dedicated to our tiny friends in the fertilizer world: micronutrients! There are quite a few; I don’t want to be a-BORON you (...yes I am really happy about that pun), so I decided to break the nutrients into two separate blog posts. This post is background of the micros, and part two will go into the nitty gritty details. When we say micronutrients, we are referring to the following elements:

  1. Manganese (Mn)
  2. Boron (B)
  3. Copper (Cu)
  4. Zinc (Zn)
  5. Molybdenum (Mo)
  6. Cobalt (Co)
  7. Some say Iron (FE) is a micronutrient. I classify it as a secondary nutrient.

Apologies in advance for all the puns related to the elements.


Not your Tomato Garden: Why Micronutrients are Important for your Hydro Garden

It’s quite amazing the number of accommodations we must make as hydro cannabis farmers. If you read my post on types of water sources, I mentioned that soil-based growing has a little bit more room for error (the soil is a “buffer” of sorts). As you can tell by the name of these nutrients, micronutrients do not need to be kept in high concentrations (often kept in the ranges of parts per million, or ppm). In fact, many of these nutrients in high quantities would be toxic. It’s just like cheeseburgers… small quantities and they’re great; too many and your doctor is going to yell at you. Anyway, usually soil typically has micronutrients already present and would not require fortification. Obviously, if you depleted the soil nutrients, you would need to supplements to get your micros.

Obviously, without soil in a hydroponic garden, you are robbed of these essential micronutrients. Thus, it is essential to make sure your plant is getting the supplemental micronutrients it needs.


“Cool Story Bro. ‘Micro’ Just Means Less Nutes, and it’s no Different than N-P-K or Ca…”

I wish it were that simple. To add more complexity, not all micronutrient sources are the same. The reason why has to do with nutrient mobility: micros tend to be very immobile nutrients. This is a good thing, because if all the heavy metals in the world were able to move quickly through the soil, we would be in trouble.

The easiest way to explain this is via the periodic table: most of the micronutrients are called transition metals (in the middle section of the periodic table). These metals have super high affinities for the charged particles in soil (or in your case, the anions that are present in your reservoir or at the root zone such as phosphates, sulfates, etc.). So instead of going into the plant, they would interact with these things, and not be taken up by the plant. The issues I’m describing can be similar to “nutrient lockout” you see with calcium or phosphorous; but at super small levels you may not even see it happening!


Chill Out… We have Metal Chelates to Save Us!

I set up the above title to hopefully make you say, “chelates” wrong (if you said “chillates”…got ya!). Chelates (pronounced key-lates) are a special way to describe these micro metals. Instead of using an iron source like iron (III) sulfate (which would likely get stuck in your reservoir and not be absorbed by the plant), iron comes in “chelated” form: Fe-EDTA, Fe-DTPA, Fe-EDHHA, etc. Chelated metals are much easier for the plant to be able to take up in a usable form (Wallace, A. et al. Soil Science 1957, 84, 27–42). An entire separate post will be devoted to this, but just know that when you look at Elite Base Nutrient A, all those “EDTA”-based nutrients are essentially the security escorts for the micros to get into the plants! Side note: not all transition metals require chelation. So, let’s get into it now!


The Iron-y: Is Iron a Micro or a Macronutrient?

Much of the world considers iron a micronutrient – as a scientist, I actually lump it into the “secondary macros” category, because its deficiencies are similar to what we see in secondary nutrient deficiencies. The take home message about iron (discussed in detail in a future post): Fe-EDTA = chelated iron = good for your plants in small quantities for most water systems. Iron chlorosis is the yellowing of the leaves with still green veins; your plant needs more iron if this happens!

That’s all for this post, stay tuned for part 2 of the microtalk!


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Want to get in touch with Elite Garden Wholesale? They can be reached via the following methods:

  1. Website: https://www.elitegardenwholesale.com/
  2. Email: info@elitegardenwholesale.com

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About the Author

Dr. NPK has been in the chemical formulating business for over 9 years. With a Bachelors in chemistry from UCLA and a Ph.D. in chemistry from UC Irvine, he has always had a passion for chemical formulation. Over the past several years, his focus has narrowed towards the research and development of products that are optimized for cannabis. He assisted in the development of the Elite Nutrient line and takes great pride in the products he created and uses himself. He has made it his mission to cut through all the pseudoscience available on the web and to educate readers on the science behind growing top-shelf bud.


Dravet Syndrome and Medical Marijuana

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In this contributor article, Amy Zukeran of Amy’s Cannabis Garden explores how cannabis can be used to treat individuals with Dravet's Syndrome. This article has been reposted and edited with permission from Amy Zukeran. The original article can be found here.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.

Dravet Syndrome is a medication-resistant seizure disorder. The causes are mostly conjecture at this point; theories range from genetic-based disorders to neurotransmission problems. The National Institute of Neurological Disorders and Strokes estimates that up to 80 percent of cases are caused by a genetic defect essential for proper brain functioning. Research has shown that Dravet syndrome patients have a mutation in a specific gene (SCN1A) which occurs in the egg or sperm before fertilization. Around two years of age, the symptoms becomes apparent in the child’s development.

Dravet Syndrome is often associated with febrile seizures – seizures associated with high body temperatures but without any other distinct cause. Triggers may include elevated body temperatures, emotional stressors, flashing lights, and excitement. Myoclonic seizures, or involuntary muscle spasms, may also occur. Some sufferers may experience seizures that last as long or longer than 30 minutes. There are many additional conditions associated with Dravet syndrome, including:

  1. Delayed growth
  2. Chronic infections
  3. Delayed language and speech development
  4. Balance and stability issues
  5. Irregular body temperature and sweating
  6. Stunted bone growth
  7. Sensory integration dysfunction

It is thought that children with the syndrome don’t produce enough of a specific protein. Those proteins would normally decrease the brain’s excitability, reducing or preventing seizures. Think of the whole system as a see-saw. The brain is constantly trying to balance between excitatory and inhibitory impulses. In cases of epilepsy, one side of the see-saw is heavier, specifically the excitatory impulses. Frequent seizures typically indicate a permanent shift toward excitatory impulses, resulting in a diagnosis of epilepsy.

Severe bouts of epilepsy and life-threatening seizures can result from this illness. One trial indicated that cannabidiol (CBD) reduced seizure frequency of FIRES, or Febrile Infection-Related Epilepsy Syndrome. At a molecular level, it is possible that CBD may modulate intracellular calcium channels of brain receptors. By slowing down this process, it is thought that nerve signal transmission is interrupted, calming down high excitatory levels and shifting the see-saw back towards a more balanced state.


Possible Cures?

CBD has been found to be anticonvulsant in mice. Scientists conjecture that CBD activates a cannabinoid receptor naturally found in the body, known as CB1. This receptor accepts endogenous cannabinoids as well as external cannabinoids, decreasing the excitability of neurons by inhibiting the release of a neurotransmitter that increases excitability.

Early research shows that medical marijuana (MMJ) may be an effective treatment for Dravet syndrome and severe epilepsy. In a self-reported survey, 84 percent of parents agreed that MMJ dramatically reduced the number of seizures in their child. Of those parents, 11 percent said child had a complete remission of epileptic seizures and 42 percent said they saw an 80 percent reduction of seizure frequency. Other studies have shown similar findings. One alarming statistic read that patients tried 12 different traditional anticonvulsant drugs before turning to MMJ as an effective remedy of various symptoms.

Currently, there are no U.S. Food and Drug Administration sanctioned drugs for Dravet syndrome. GW Pharma, a U.K.-based company, has developed a high CBD medicine, called Epidiolex, which has been shown to ease seizures in patients with this illness. It is a plant-based derivative that has also been shown to possibly decrease neural transmission and excitability. CBD-based compounds are currently in stage 2 and 3 research trials for use in the U.S. Depending on the outcome of these trials, these extraordinary compounds are a few years away from approval. Let us hope.


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  1. Website: http://amys-cannabis-garden.com/

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About the Author

Amy was born and raised in the Puna Bud Capital of the World -- Hawai'i. She is genetically programmed to write and also explore medical cannabis so visit her blog. Mahalo Nui Loa!


Maintaining Growroom Yields During Drought: Part II

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In this series of contributor articles, Clif Tomasini of Quest Hydro continues the discussion on how to maintain a proper grow room with great yields despite an ongoing drought. With concerns about climate change in the future, these tips may come in handy!

Want to read Part 1? Click here to read it.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.


Disclaimer

This article has been reposted with permission from Quest Hydro. The original article can be found here.

Regardless of Drought

Dehumidifiers are essential to healthy yields, because they help maintain optimal moisture levels in grow rooms. They can also solve the drought-induced water scarcity so many growers face: Dehumidifiers can provide you with a source of clean, readily accessible water even during drought.


Dehumidifiers can Give you a “Free” Renewable Supply of Water

A dehumidifier is basically a refrigeration system. It has two copper tubing coils; each coil looks similar to an automotive radiator. One of the coils is hot, and the other is cold.

The system works by pulling heat from the cold coil and “exhausting” it through the hot coil. A fan blows air through the system, which removes water from the air and heat from the hot coil. The water condensate forms on the cold coil, like condensate will form on a glass of ice water during a hot day.
The condensate then runs off the cold coil and is collected into a reservoir.


Dehumidifier Water can be Preferable to Reverse-Osmosis Filtered Water, thanks to the “Multiplier Effect”

Even without a drought, it’s preferable to use the perfectly good water that dehumidifiers produce rather than “dump” it – and it can actually be better than reverse osmosis water. Why? Condensate water from dehumidifiers is naturally clean and free of contaminants, while reverse osmosis machines must necessarily waste 1 to 3 gallons of water to produce a single gallon of clean water. Every gallon of dehumidifier condensate saves 2-4 gallons of water drawn out of the tap.


Is the Condensate Water Clean and Free of Contaminants?

Yes. The cold condensate coil is enclosed within the dehumidifier. Because dehumidifier coils are not soldered with lead as is common in other applications, there’s no chance of lead contamination, either. Instead, copper coils are brazed.

If you would like to read part one, click here.


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  1. Website: https://questhydro.com/
  2. Email: info@questhydro.com
  3. Phone: 877-420-1330

Do you have any questions or comments?

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About the Author

Quest builds quality, energy-efficient dehumidification equipment designed to perform in commercial environments. Quest has the solution for any indoor or greenhouse gardening application. Quest dehumidifiers are extremely energy efficient on the market, removing the most pints of water per kWh over the widest temperature range.


Using Light to Repel and Attract Insects

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Gary Sigman of Metaphase Lighting Technology has observed behavioral responses from insects in reaction to certain colors of light. He writes about experiments with lighting technology here.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.

Editor’s Note: There are several research sources that have discussed the phenomenon of phototaxis. Before buying or installing equipment, make sure to do your research on what insect pests you’re hoping to repel or attract. Growers Network always recommends conducting your own experiments before taking anyone’s word as truth!


Phototaxis

I had an interesting experience early in my career at a warehouse in Texas. The facilities manager decided to switch from HPS lighting to metal halides to take advantage of their color rendering index. Within months, the manager noticed that there was a spider problem.

When the manager called me, I realized that the yellow lights had previously been keeping the spiders out. After some painful modifications, the manager switched back to the HPS lights, and, according to what he told me, within minutes spiders were marching down the aisles of the warehouse and out the doors. Within a few days, all the spiders had vacated the building.

This is when Metaphase began developing its own insect-deterrent light.


Repulsion


Editor’s Note: Lights may attract or repel insects depending on the species. For example, it is well-known that moths are attracted to blue light, or that cockroaches are repelled by virtually any light. Every light may have a tradeoff as to what insects it attracts and repels.

Aphids

A Midwestern university tested our experimental insect deterrent light. They found that it was most effective on aphids, as they would evacuate a growth chamber in which the light had been placed. The chamber was completely bug-free within the week. I followed up the university tests with experiments in cannabis greenhouses located in Colorado and Maine. Aphids consistently moved away from areas illuminated by the lights. This was observed by Tuinier Brothers Flowers when they positioned their hanging baskets, infested with aphids, between the lights.

Mites

The same university also was the first to notice that our lights deterred spider mites. Mites would not cross from infested plants to nearby healthy plants that were illuminated by the insect deterrent light. It was as if the light acted as a barrier that the spider mites would not cross. We took this knowledge and field tested the lights in greenhouses of Colorado and Maine that had already had an infestation of spider mites. The lights were positioned on the floor beneath the canopy of the cannabis. The mites hid on the top of the leaves to get away from the light and laid their eggs on non-illuminated plants.

Thrips

Again, the same university also reported that the light was keeping thrips out of illuminated grain. This grain was even placed inches away from infested plants. The thrips simply stayed away from the light.

Colorado Russet Mites

Brighterside Vertical Farms in Colorado reported that their customers had been experiencing outbreaks of russet mites. They wanted to try the insect lights to see if they would have deter mites that were infesting some cannabis clones. We saw two results:

  1. The russet mites ran away much faster than expected.
  2. The clones grew much healthier stems and thicker and longer roots. Whether this was a side effect or direct cause is inconclusive.


Attraction


Editor’s Note: Lights may attract or repel insects depending on the species. For example, it is well-known that moths are attracted to blue light, or that cockroaches are repelled by virtually any light. Every light may have a tradeoff in what insects it attracts and repels.

Japanese Beetles

A grower I worked with had an infestation of Japanese Beetles. Much to his surprise, the beetles were flying into the light that he was using to repel mites. The beetles would fly into the light and then fall to the floor of his greenhouse. In a clever move, he placed a bucket of water where the beetles were falling. After that, his concern became changing a bucket, not managing an infestation.

Whiteflies

Consejo Superior de Investigaciones Científicas, a Spanish agricultural research center, found that the insect light was very effective on whiteflies, but not in the way they were expecting. The research personnel thought that the whiteflies would be repelled by the light, but to their surprise, the whiteflies flew toward the lights. The researchers attached some double-sided film tape over the lenses of the lights, and the whiteflies flew into the tape. After applying some clean tape, the researchers were able to capture and remove each one of a hundred whiteflies.

Psyllids

A US government research facility was looking for a solution to fight psyllids (jumping plant lice). The researchers expected that the psyllids would be repelled from the light, but instead the psyllids were attracted to it. The researchers set up a test area which included a window to the outside (daylight) and a translucent panel located 4’-6’ away illuminated with the insect light. A plant was placed about 6 inches from each panel. The 200 psyllids, which were reared at this facility, were released in the middle of the test area. More than 60% of the psyllids flew toward the insect light while the others ventured towards the daylight. The psyllids that headed for the daylight landed on the plant, while the psyllids that headed toward the insect light flew right past the plant and landed on the illuminated panel.


Future Tests

We’ve scheduled our insect deterrent light for additional tests throughout the world. The light was recently made available for commercial use, and can be found on our website.


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Want to get in touch with Gary Sigman? They can be reached via the following methods:

  1. Website: https://www.metaphase-tech.com/
  2. Email: G.sigman@metaphase-tech.com

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About the Author

I have been in the lighting business for over 20 years. Recently I became the Business Development Manager for the Metaphase Lighting “New Technology” products. I am regularly in contact with growers, researchers, and scientists throughout the US and the world.


Variations in Cannabinoid Reporting: Part Five

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In this series of contributor articles, Savino Sguera of Digamma Consulting explores the reasons that laboratories may report different cannabinoid and terpene profiles for similar samples. There are many steps in sample testing and collection that can go wrong or can be intentionally manipulated, and Savino is here to guide us through the process.

Want to read the fourth article? Click here.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.

Based on a presentation to the American Chemical Society Fall Conference, 2016

by Savino Sguera


Introduction

Savino Sguera of Digamma consulting continues his analysis on the reasons why cannabinoid and contaminant reporting can vary heavily in the cannabis industry. Click here to see part four!


Sample Size and Homogenization

Sample size is the amount of flower that is taken for a given analysis from total submitted sample. When considering sample size, larger samples generally reduce the effects of variation, as localized variations in the sample will average together. Digamma recommends that laboratories begin with a minimum sample size of ½ a gram or 500 mg of flower, with 1 gram or 1000 mg for greater accuracy. At times performing the analysis twice with two different 500 mg samples is also desirable, as it will show the relative deviation between the two analyses. This measures the test’s precision. Labs that use less than 500 mg sample size encounter significant variability.

Figure 7: A graph demonstrating how precision of a given test (y axis) changes based on the the mass of sample used (x axis).

Figure 7 shows a graph relating cannabis flower sample size to relative precision. The lower mass samples, such as 100 mg and 300 mg, show significant variability between one analysis and the next, with variations as high as 50% and 10% respectively. Once we reach approximately 500 mg of cannabis flower, precision starts to achieve the desired precision of 2% or less. Past 500 mg, we can see the range becomes very narrow, and by 1000 mg a very accurate and precise number can be reported for a sample.

Small sample sizes can be used to intentionally manipulate lab results as well. If a very small sample size is tested in triplicate, the lab can release the most desirable result and gain a substantial change in the reported value. In contrast, if a large sample size is used, the range of results will be very small, and manipulation will be difficult.

Figure 7 assumes that the cannabis flower has been thoroughly homogenized. Precision becomes an issue even at high sample sizes if the sample is not properly homogenized. Homogenization breaks the flower sample into very tiny pieces, mixing them evenly, without loss of chemical constituents or contamination by outside sources. Homogenization is a necessary step before chemical analysis, but homogenization quality can have a large impact on the final lab results.

Cross-contamination is a critical issue in sample homogenization. Cross-contamination is a process by which a contaminant, such as a pesticide or mold, from a sample contaminates a different sample. Good laboratory practices and proper procedures should preclude the possibility of any cross-contamination. If the proper homogenization technique is not used, it is very easy for grinding equipment to carry contaminants from one sample to another.

Figure 8: A presentation of three sample homogenization techniques. Techniques highlighted in green have these attributes, those highlighted in red do not.

Figure 8 outlines different sampling options for the homogenization of cannabis. From left to right, the first illustrated option submerges the sample in inert liquid nitrogen and grinds it with a mortar and pestle. The second option uses a mechanical grinder (such as a blender) to shred the cannabis flower, and is popular with cannabis users. The third option uses a laboratory-grade tissue homogenizer in a disposable sample tube, with an acetone bath between uses. We judge the three options by three criteria:

  1. Does the homogenization method prevent the degradation of cannabinoids and the evaporative loss of terpenes?
  2. Does the homogenization method homogenize to a very fine particle size?
  3. Does the homogenization method prevent cross-contamination between samples?

It is worth noting that the reason the aforementioned liquid nitrogen technique fails at preventing cross-contamination is because the mortar and pestle house microscopic pores. There are liquid nitrogen homogenization options that use stainless steel balls to grind the sample. Stainless steel balls can be industrially washed on a routine basis. Such homogenization techniques prevent cross-contamination and are an appropriate option for the analysis of cannabis.


Moisture Content

Moisture content is the amount of water in the sample. Moisture content is normally measured in percent of mass and properly-dried cannabis is somewhere between 7% and 12% moisture content. Fresh, uncured cannabis is close to 90% moisture content. This means that after harvest, 100 lbs. of fresh cannabis may weigh only 10 lbs. when cured and ready for consumption.

Moisture content can be measured in numerous ways. The Karl Fischer titration (abbreviated as KF) is perhaps the most accurate method, as it chemically absorbs every water molecule in the sample and measures it according to a very strict reaction. Though highly accurate, this method is very expensive and impractical for cannabis laboratories.

In most cannabis labs, moisture content is typically measured with a simple laboratory technique known as Loss on Drying (LOD). This technique is used in environmental analysis industry to measure soil moisture content and in the food industry to measure moisture content of food. Loss on Drying forces the loss of moisture in a sample via heating in a convection or vacuum oven. The mass of water in the sample is deduced by massing the sample before and after drying. This approximation depends on the absence of volatile compounds such as terpenes, but it is a good enough approximation for cannabis flower to be practical.

Figure 9: A diagram illustrating the moisture content of cannabis and how it affects final reporting results.

In Figure 9, we can see how reporting THCA in the sample as it was received or dried has a noticeable effect on the final reported number. Reporting based upon as-received weight gives an accurate THCA amount that is useful in dosing and deducing the bulk amount of THCA in a given package. Dry weight analysis provides a better comparison between products whose moisture contents may differ. While neither method is more “correct” than the other, it is important to know which method was used and that all labels contain the same information for comparison. Selective reporting on dry weight will always make a number look higher, and reporting as-received will make it look lower.


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Want to get in touch with Savino? He can be reached via the following methods:

  1. Website: https://www.digammaconsulting.com/
  2. Email: savino@digammaconsulting.com

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About the Author

Savino Sguera is founder and CSO of Digamma Consulting. Since 2010 he has been an analytical chemist and researcher in the cannabis industry, working with both private and public interests to bring scientific integrity to the business. Savino holds a B.Sci. in Biomedical Engineering from Columbia University.


Gentleman Toker – Pure Ratios CBD Reservoir Patch

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Gentleman Toker reviews CBD patches from Pure Ratios. Let's see what he has to say! The original article can be viewed here.

Joe Tierney

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. Any opinions expressed in this article do not reflect the opinions of Growers Network. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.

Quick Look

  • Pros

    Strong, almost immediate relief for muscle pain.

  • Cons

    Didn't quite make it to the advertised 96 hours, but 60 hours of relief ain't bad!


Whether it’s a long drive down old country roads or flights across the country, your Gentleman loves to travel. More than that, I need to be mobile to keep up with all my brand meetings so I can share my neurotic nematodes info on the newest, hottest cannabis gifts around! Having a literal pain in my ass that cranks up from sitting down (Editor’s Note: Don’t google this.) has made travel incredibly restrictive. I don’t usually drive more than three hours total on my best days, so I was freaking out about the six hour flight to Vegas. It sucked, despite a nearly vacant redeye allowing me an entire row to stretch out as best I could. Already dreading the flight back, I hit the Marijuana BizCon with an eye out for topical solutions that might provide some relief and was amazed with how well the Pure Ratios CBD Reservoir Patch worked.

Editor’s Note: If you live in Washington DC, Gentleman Toker finagled a deal with Canamelo delivery service. Check the original article for the details.

Pure Ratios makes CBD topicals, patches, lozenges, and vape cartridges that can be purchased from their website and shipped nationwide. Their full product list includes items containing THC, but those aren’t available for shipping. I’ll be looking for them when I make it out to Cali! Pure Ratios utilizes a proprietary CO2 extraction process of the whole hemp plant to produce their full-spectrum, terpene-rich hemp oil, which is third party lab-tested for potency, pesticides, and microbiologicals. Legit! They toss some other herbs in there for good measure- I’m very interested to see they have vape cartridges that include the anti-inflammatory turmeric, for example.

Editor’s Note: Get your mind out of the gutter.

The Pure Ratios CBD Reservoir Patch I tried was the same one available for shipping online, a 40mg dose. I slapped a patch on my aching butt post-shower Thursday morning and was feeling some relief within the first few minutes. It took about forty minutes to reach its full effectiveness and I definitely noticed when it did. I felt fantastic! When you live with chronic pain, any time off makes you feel like running outside in a bright blue tux, singing to strangers, but if you try this in Vegas the street performers will think you’re stepping on their turf and you’ll be chased back to your hotel by a fat Jon Snow and a topless nun. Fair warning!

Thanks to Pure Ratios patch, I spent Thursday relatively pain-free, including the flight home. This time, the plane was packed. Despite having no extra room to work my leg into a good angle, the trip back was much more bearable. And the patch was still kicking the next day! Pure Ratios touts up to 96 hours of relief and I’d say I got around 60 - the pain was back when I woke up Saturday morning. And these things are waterproof so you don’t have to choose between losing a still-good patch or smelling like sewer-fight Batman! The Gentleman is hooked. I’m gonna get some more. A lot more. After the success of this patch, I’m really excited to try out the rest of Pure Ratios menu, especially the lozenges. Check ’em out for yourself at their website!


Editor's Note: Want to know even more about CBD? Check out TruPotency, the only online retailer that 3rd party tests every product they sell!



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  1. Website: http://www.gentlemantoker.com/
  2. Email: joe@gentlemantoker.com

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About the Author

Joseph Tierney is an independent, award-winning cannabis blogger based in Washington, DC. Well, OK, the awards are hypothetical at this point because there aren't any for horror/comedy/scifi-based internet rants from a dabbed-out misanthrope, but if there were, GentlemanToker.com would win them all. But, like, probably not, cuz politics. Right? You feel me.


Optimize Your Grow for a LED Lighting Strategy – Part One

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This is part one in LumiGrow’s series for measuring and managing your room temperature, relative humidity, carbon dioxide concentration and light intensity. Learn more about LED lighting strategies on their website.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.


Part 1 - Ambient Temperatures and CO2 Concentrations


Overview

The newest generation of powerful LED grow lights have changed the way that growers maximize profits and crop quality. Growers often ask what adjustments need to be made to their growing environment in order to optimize their facility for LED lighting. Dr. Matt Wheatley, a cannabis consultant working with LumiGrow, explains:

“New and experienced growers that want to reap the benefits of an LED strategy should familiarize themselves with the relationships between environmental factors that affect the efficiency of photosynthesis; leaf temperature, humidity, carbon dioxide concentration, and light intensity. In this article, we’ll discuss the effect of temperature on photosynthetic rate; how temperature and CO2 concentration are intimately linked to plant growth at higher temperatures; and compare the differences between ambient temperatures in HPS and LED grow rooms,” explains Dr. Matt Wheatley.


Leaf Temperature vs Ambient Room Temperature

When scientists discuss photosynthesis and temperature, they typically reference leaf temperature, not the ambient room temperature; this makes sense given that the biochemistry of photosynthesis takes place inside plant’s leaves. In contrast, when grow room designers discuss temperature, they usually reference the ambient room temperature. In most growing environments, the leaf temperature will be higher than the ambient air temperature surrounding the plant. This is especially true for plants grown under high-pressure sodium (HPS) lights, which emit infrared radiation that is absorbed as heat by the plant.


Temperature Requirements for Photosynthesis

RuBisCO is the plant enzyme responsible for the chemical reaction that is the first step of carbon fixation. This chemical reaction is seen as the conversion of CO2 and water into simple sugars during photosynthesis. The chemical reaction that RuBisCO performs is temperature dependent.

With full sunlight and ambient CO2 concentrations of about 300ppm, as well as a temperature range of 5°C-27°C, the rate at which CO2 is absorbed by the plant and converted to sucrose increases as the temperature increases (Figure 1 below), leading to increasing gains in net photosynthesis.

If the internal leaf temperature rises above 27°C, RuBisCO enzymes begin to perform the reverse reaction, with some of the RuBisCO enzyme converting sucrose and oxygen into CO2 and water in a process known as photorespiration. As leaf temperatures approach 40°C, net photosynthesis will become negative as the plant burns more carbon than it gains. So, under normal ambient CO2 levels, a grower will achieve the greatest growth with leaf temperatures just below 27°C.

Fortunately, an indoor grower can adjust their environment to achieve optimal growing conditions. Controlled environments allow growers to maintain optimal temperatures, carbon dioxide concentrations, light intensity and relative humidity. So, let’s explore how a grower can adjust the growing environment to take advantage of high rates of growth that occur at high temperatures.


The Importance of CO2 Enhancement for High Temperature Growing

Increasing CO2 concentrations will extend the temperature range in which RuBisCO may fix CO2 into sugar. With increased CO2 we see that as temperature increases, so does the rate of the chemical reaction that RuBisCO performs. This works because an increase in CO2 concentration means that the ratio of chemical substrates to products is being increased. If the CO2 concentrations are increased from ambient 300ppm to 1500ppm, the change in the ratio of reactants to products will allow plants to continue to fix CO2 into sucrose at leaf temperatures well above 27°C, all the way up to about 36°C! (see Figure 1)


Figure 1 - Changes in photosynthesis as a function of temperature at CO2 concentrations that saturate photosynthetic CO2 assimilation (A) and at normal atmospheric CO2 concentrations (B). Photosynthesis depends strongly on temperature at saturating CO2 concentrations. Note the significantly higher photosynthetic rates at saturating CO2 concentrations. (Taiz and Zeiger, Plant Physiology 3rd edition)

And as the temperature increases, so does the rate of carbon fixation and plant growth. This means that if growers are careful with their environmental controls, they may achieve very high rates of carbon fixation and plant growth at leaf temperatures well above 30°C.

[Pro Tip]: Consider adjusting the vapor pressure deficits (VPD) in your grow spaces for the various phases of plant growth to see optimal crop performance.

Related Article: VPD for Cannabis Cultivation


Infrared Radiation Creates Leaf Temperature Gradients Down the Canopy

HPS bulbs emit a large infrared peak between 800nm and 900nm. This infrared peak significantly increases leaf temperatures at the top of the canopy, where most of the infrared light is absorbed. When examining the differences between leaf temperatures of plants grown in the same room under either HPS or LED lights, we will see significant temperature differences that infrared light causes. Figure 2 shows the difference in leaf temperature for leaves 3’ below either LED or HPS lamps in the same room with the ambient temperature set at 25.7˚C.

Figure 2 - The effect of Infra-Red light on internal leaf temperature. (Graph courtesy of LumiGrow Plant Research Group)

In one of our studies, cannabis consultant Dr. Matt Wheatley measured the photosynthetic activity and internal leaf temperature of leaves at different distances from the light source. The internal leaf temperature measurements were very clear. The leaves of plants under LEDs did not show an increase for internal leaf temperature significantly above the ambient room temp at any distance between 2’ and 4’ from the lamp. In contrast, the leaves of plants under the HPS lamps showed a wide range of internal temperatures; the highest temps were apparent at the top of the canopy and lowest internal leaf temperatures were at the bottom of the canopy. This partially explains why HPS lamps produce top-heavy crops while LED lighting creates a more uniform canopy.

Since the rate of carbon fixation by RuBisCO is affected by leaf temperature and CO2 concentration, increasing the ambient temperature in LED-lit rooms will increase the rate of photosynthesis and plant growth.


Raise the Temperature in an LED Room

Based on scientific study and experimentation, LumiGrow has found that an increase in the ambient room temperature of 5-7°C in LED-lit rooms relative to temperatures in HPS grow rooms is necessary to achieve similar internal leaf temperatures and plant growth rates as those experienced by plants in HPS-lit rooms. Keep in mind that as the ambient room temperature increases, the relative humidity decreases, and proportionate adjustments should be made to the relative humidity to adjust your Vapor Pressure Deficits.


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  2. Phone: 800-514-0487
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About the Author

Dr. Matt Wheatley brings significant industry experience as a cannabis cultivation consultant and molecular biologist. Matt earned his BS in Botany at WSU, and a PhD in Biochemistry and Molecular Biology from UNR in Reno, NV. Matt currently works as a cannabis consultant for LumiGrow, a smart horticultural lighting company, where he discovers plant and light interactions and develops strategies for optimizing profits and yields.


HIV/AIDS and Medical Cannabis

In this contributor article, Amy Zukeran of Amy’s Cannabis Garden discusses how cannabis can be used to treat HIV and AIDS-related symptoms and syndromes. This article has been reposted and edited with permission from Amy Zukeran. The original article can be found here.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.

Remember the massive AIDS scare? It almost seems like it happened ages ago. When I worked with gay guys in the 80s who were either HIV positive or had full-blown AIDS, it was a death sentence. You don’t know how sad it was hearing a 25-year old coughing with pneumonia from AIDS. Knowing it was a death sentence was terrible. The worrisome thing is that 1 in 8 don’t know they are HIV positive. Worse yet, it could take some people ten years to show any signs of HIV.

First, some definitions:

  1. HIV, or Human Immunodeficiency Virus, is a virus that spreads through bodily floods, including semen, blood, and milk. HIV targets immune cells, making the body vulnerable to opportunistic infections and leading to AIDS.
  2. AIDS, or Acquired ImmunoDeficiency Syndrome, is a syndrome characterized by a lack of immune response to infections. These infections, which would normally be harmless to a healthy person, become deadly.

Truth be told, HIV and AIDS have never left us, but have instead become a footnote in the western world because of the spectacular success of antiretroviral cocktails which slow or halt the progression of HIV. They have lowered the mortality rate and extended lives.

However, not everyone has access to STI screening, or antiretroviral meds. By the end of 2015, there were 36.7 million cases of AIDS and HIV worldwide. Since the the Western world first learned about the virus in June of 1981, there have been 35 million deaths worldwide. There are currently 1.2 million people living with AIDS in the U.S., and over 650,000 have died. There are typically 50,000 cases of HIV a year in this country. It is thought that there is about 7 million people worldwide that is HIV positive.

Even if you have access to the antiretroviral drugs, sticking with the drug regimen can be difficult. Common reactions to antiretroviral drugs are nausea, vomiting, and appetite and weight loss. AIDS becomes a wasting disease, instead of a lethal one.


Enter Cannabis.

It is estimated that one in three AIDS patients use cannabis for symptoms such as nausea, appetite loss, pain, and cachexia (weight loss that cannot be reversed by eating more). Many studies have shown the efficacy of cannabis to alleviate nausea, weight loss and appetite loss. It is also useful for treating neuropathic pain (pain originating from the nervous system). Patients who use cannabis along with their AIDS cocktail medications are just as likely to stay with the drug regimen as non-users, but experience fewer symptoms.


HIV Rears Its Ugly Head; Research Fights Back.

HIV infections are on the rise as another generation forgets the lessons of its elders. There are ever-increasing reports of young, gay men finding themselves HIV positive. There is also a rise in heterosexual infections recently, which are typically tied to the rise of HIV in the bisexual population.

In spite of all this, there have been advancements in research for treatment. One 2012 study by Mt. Sinai School of Medicine found that a cannabinoid compound can block the mechanism which allows HIV to enter and infect healthy cells. There is a recent discovery that a compound of cannabis, Denbinobin, was mostly responsible for inhibiting HIV replication. That’s big news for AIDS researchers.

Another possibly important discovery pertains to some cannabis compounds and their interaction with the brain. A study by Temple University researchers found that cannabinoid 2 (CB-2) agonists can stop HIV-Associated Neurocognitive Disorder (HAND), a disorder caused by HIV. Most antiretroviral drugs cannot pass through the blood-brain barrier that protects the brain, but cannabinoids can.


With about 94 percent of Americans supporting the use of medical marijuana for various ailments, research dollars should be pouring in. Sadly, with this uncertain political climate, your guess is as good as mine as to whether there will be a place for cannabis in a physician’s bag. Hope for the best. Aloha.



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About the Author

Amy was born and raised in the Puna Bud Capital of the World — Hawai’i. She is genetically programmed to write and also explore medical cannabis so visit her blog. Mahalo Nui Loa!


Ask DryGair: Ventilation vs. Dehumidification

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What are your HVAC needs? What do you want to know about the dewpoint? In this series of contributor articles, Yonatan Peretz and Hadar Fuchs-Rubal of DryGair want to answer your questions about climate control inside your growing facilities or grow operation.

Hadar Fuchs-Rubal | Yonatan Peretz

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.


Ask DryGair: An eMail-In Question and Answer Series


Ventilation vs. Dehumidification

Last month our topic was humidity. This time we’ll discuss ventilation, a traditional, if inefficient, method to reduce humidity.


How can you solve humidity problems with ventilation?

Growers in closed facilities face complicated challenges, one of the more complicated challenges being humidity.

Humidity inside a growing facility is generally caused by transpiration from plants. If humidity gets too high, drops of water form on the plants, causing humidity-related diseases such as Downy mildew, Botrytis, Alternaria, Powdery Mildew, Erwinia, Phytophora, or Clavibacter. Reducing the humidity inside growing facilities can prevent diseases and reduce the need for pesticides.


Then how can we reduce humidity indoors?

A traditional solution to treat humidity is ventilation. Ventilation is a relatively low-tech solution to humidity issues.

There are two main types of ventilation:

  1. “Natural” ventilation is the opening and closing of a growing facility’s doors and windows to replace the humid air inside with external (hopefully) dry air.
  2. “Forced” ventilation uses fans to replace the humid air inside with external (hopefully) dry air.

Plants transpire all day. They transpire around 88% of the water they absorb during the day. When the weather outside is dry, ventilation can be a very effective method to reducing humidity. Moreover, during daytime (when it’s warmer) ventilation can also lower the heat inside.


What happens during the night or when the weather is not good?

There are three main problems growers face during nights or on cloudy, rainy, or cold days:

  1. Loss of energy. Opening a growing facility leads to loss of warm air, especially in colder conditions. A loss of heat energy often leads growers to use more energy in order to reheat the facility. In fact, according to experts, ventilation can be calculated as an average energy loss of around 75kw.
  2. Moreover, when the air outside is just as humid, or more humid than the air inside the growing facility (on rainy days, cloudy days) ventilation becomes ineffective.
  3. No uniformity. Taking in external air creates microclimates of temperature and humidity inside the growing facility. This difference in climate conditions affects the growing process and the development of the plant, ultimately leading to an inconsistent yield.

Editor’s Note: Colder, humid air can be brought in as well. Because the air is colder, it contains less water, but can have the same RH. If the cold, humid air is heated up, it lowers in RH. This is relatively inefficient method of dehumidification, but is a ventilation method of dehumidification.

These days, many growers are acknowledging the importance of dehumidifiers. DryGair products, for example reduce the need for ventilation during the night or on cloudy/rainy days. The goal is humidity reduction without ventilation. This can save upwards of 50% of the energy invested in the operation of the facility.

Please use our contact information below to send us questions!


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  1. Want to get in touch with DryGair? They can be reached via the following methods:
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    2. Phone: +972-9-7730980
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About the Author

Hadar Fuchs-Rubal is DryGair's Economist and Marketing Manager. Hadar specializes in agriculture and environment economics. Her experience includes economic and business consulting for the private and public sectors - mainly on environment and agriculture subjects.


Variations in Cannabinoid Reporting: Part Four

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In this series of contributor articles, Savino Sguera of Digamma Consulting explores the reasons that laboratories may report different cannabinoid and terpene profiles for similar samples. There are many steps in sample testing and collection that can go wrong or can be intentionally manipulated, and Savino is here to guide us through the process.

Want to read the third article? Click here.

The following is an article produced by a contributing author. Growers Network does not endorse nor evaluate the claims of our contributors, nor do they influence our editorial process. We thank our contributors for their time and effort so we can continue our exclusive Growers Spotlight service.

Based on a presentation to the American Chemical Society Fall Conference, 2016

by Savino Sguera


Introduction

Savino Sguera of Digamma consulting continues his analysis on the reasons why cannabinoid and contaminant reporting can vary heavily in the cannabis industry. Click here to see part one!


Sampling Errors

Sampling is the process of of taking a representative sample of a product for chemical testing. In environmental laboratories, sampling is frequently performed by a certified third party sampling service, trained in the methodology of proper sample collection. These samples can be used for the detection of lead in drinking water or a mold outbreak in a residential property.

However, in the cannabis industry, the process varies by state jurisdiction. In Colorado, Massachusetts, and formerly California, cultivators or dispensaries drop off cannabis samples at the lab without any formal training. In Nevada and now California, the lab must go to the cultivation or manufacturing site and sample from their final cured product. Although no state has yet mandated third party sampling services, it would greatly aid in the precision and alignment of results between laboratories in that jurisdiction as it has in environmental, food, and drug testing.

The best sampling method can be determined by a statistician; many international shipping and large scale manufacturing companies employ them. One of the most practical approaches is random sampling, where samples are taken randomly from within the batch. This guarantees that while variation may occur from sample to sample, statistically the overall results are very close to the actual batch average. When intentional manipulation is afoot, selective sampling is often employed. This occurs when the sample is carefully selected from the whole of the batch to influence certain values in the lab report, such as maximizing cannabinoid potency or minimizing mold spore counts or pesticide levels. Below I will illustrate some of the different ways in which a sample may be biased.

Figure 5: Photographs of mature cannabis plants ready for harvest with illustrated potency gradient increasing toward the top.

Cannabis plants naturally have a gradient within them, with increasing THCA concentrations near the top of the plant. Figure 5 illustrates the cannabis plant’s potency gradient and distribution. As these buds are combined together into large batches, the differences should mix randomly and evenly. If buds from the top of each plant are placed into a separate container from other buds, it could be easily used to maximize the lab’s reported potency. This can be done with samples that are sent to the lab or the lab’s sampling technician fails to properly collect the samples.

Another critical issue is sample size. In Nevada, a 12 gram cannabis flower sample size has been written into the adopted regulations governing the medical cannabis businesses. In other less-regulated states, the sample size submitted is determined by the laboratory. Although labs can produce results with as little as 0.5 grams of cannabis flower, a larger sample size generally produces more precise and accurate results by assuring that the results are averaged across a large-enough amount of cannabis. A larger sample also allows for re-testing of the same sampled batch, should the need arise.

Sampling is not just performed in the field. When a 10 gram sample is sent to an analysis lab, technicians and chemists use smaller samples for each test. These samples should be also be randomly selected from the available product, weighed, and prepared for analysis. However, there is potential for a lab to influence the result via sampling. Labs can process cannabis flower to different degrees before testing for potency. This will influence or change the lab results and will inflate the reported lab’s values away from a true representation of the batch of flower in question.

Figure 6: Diagram showing three different sample preparation methods. Notice the increase in final potency with the increase in extent of processing. For consistent results, labs should follow a procedure with minimal sample manipulation.

Figure 6 shows a diagram comparing three different cannabis sample processing techniques, and how they influence the final number reported by the laboratory. This also illustrates why it is better for a third party or laboratory to collect samples, rather than rely on material sent by cultivators.

The first, and most accurate method, is testing the sample as received from the cultivator. Processing the sample before testing and removing things like stems at the laboratory will increase the final potency, but will push the reported result away from an accurate representation of the batch of cannabis flower in question. Further processing, as seen in Cannabis Sample Prep 3, involves grinding the product and filtering it through a sieve or kief screen, as discussed in the AHP guide. This removes large amounts of plant material with low THC content and causes the final reported number to be significantly higher, but moves the reported results very far away from what is representative of what the end consumer will receive.


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  1. Website: https://www.digammaconsulting.com/
  2. Email: savino@digammaconsulting.com

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About the Author

Savino Sguera is founder and CSO of Digamma Consulting. Since 2010 he has been an analytical chemist and researcher in the cannabis industry, working with both private and public interests to bring scientific integrity to the business. Savino holds a B.Sci. in Biomedical Engineering from Columbia University.