High-Performance Liquid Chromatography (HPLC)

High-Performance Liquid Chromatography (HPLC) is a chromatography technique designed to separate, identify, and quantify components in a mixture. A pressurized solvent is passed through a column containing the sample and an adsorbing material. Because every molecule in the sample behaves differently to the adsorbent material, the molecules will separate out as they move through the column.

Once the molecules are separated, they can be identified out via a number of different means, including GC-MS and spectrometry.